About LY2090314
The FLIPR assay was carried out on FDSS/µMobile with ligand addition at ten s for the binding assay and mAb1/isotype addition at 10 s accompanied by hCCL1 addition at three hundred s for that Opposition binding assay, with a complete checking of 500 s. The excitation and emission wavelengths were set at 485 nm and 525 nm, respectively.